It is important that all users can access applications built using Dojo. People with disabilities access the Web in many manners. Some people rely on the keyboard only to navigate and make selections. Others use custom font and color settings, custom style sheets, screen magnification or screen readers to interact with the computer. With the addition of multimedia to the Web, captioning is important for folks with hearing loss. Those with cognitive disabilities may use a variety of assistive technology to adapt the content to a particular learning style.

In addition, many countries require that Web content is accessible to all. Section 508 of the Disabilities Act requires the US Government to purchase accessible technology. Australia, Japan, Great Britain and the European Union all have accessibility regulations. Thus, it is important for the Dojo Toolkit to provide a way to build accessible applications.